mass spectrometer 4800 maldi tof Search Results


jasco ft ir 4800 spectrometer  (JASCO Inc)
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energy dispersive x ray spectrometry  (Hitachi Ltd)
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Energy Dispersive X Ray Spectrometry, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abi 4800 proteomics tof/tof analyzer  (AB Science)
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maldi-tof tof 4800  (Micromass UK Limited)
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Maldi Tof Tof 4800, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4800 maldi-tof/tof mass spectrometer  (PerSeptive Biosystems Inc)
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ab 2813911 testosterone xevo tqs tandem mass spectrometer  (Bio-Rad)
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voyager system de str 4800 mass spectrometer  (Thermo Fisher)
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Thermo Fisher voyager system de str 4800 mass spectrometer
Voyager System De Str 4800 Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fourier transform infrared ftir 105 spectroscopy  (JASCO Inc)
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electron microscopy  (JEOL)
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fluorescence spectrometer slm model 4800  (SLM Aminco Instruments Inc)
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4800 maldi tof/tof analyzer  (Shotgun Proteomics company)
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Shotgun Proteomics company 4800 maldi tof/tof analyzer
Ovarian cancer cell secretome increases HOMEC cellular responses and contains a range of potential proangiogenic proteins. (A) Proliferation of HOMECs treated with CM from SKOV3 and A2780 cells for 24, 48, or 72 hours assessed using the WST-1 assay. Controls received basal medium only. **P ≤ .01 and ***P ≤ .001 versus control levels (100%). (B) Migration of HOMECs in co-culture with ovarian cancer cells and HDFs (co-culture control) assessed by the ThinCert migration assay. After 24 hours, fluorescence of migrated cells was quantified. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus HDFs. (C) HOMEC Oris cell migration after 48 hours in the presence of VEGFA165 (20 ng/ml) ± SU5416 (10 µM). Controls received medium alone. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus VEGFA165. (D) Migration of HOMECs in co-culture with ovarian cancer cells ± anti-VEGFA antibody (500 ng/ml) or SU5416 (10 µM) assessed by the ThinCert migration assay. ***P ≤ .001 versus control (100%), **P ≤ .01 versus control (100%), and NS versus A2780 or SKOV3. (E) VEGF- and EOC cell-induced tube-like structure formation of HOMECs and the effects of the SU5416 inhibitor. HOMECs were plated onto fibrin matrices and exposed to either VEGFA165 or EOC cells in co-culture ± SU5416 (10 µM). Negative controls received medium alone. Tube-like structure formation was quantified. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 versus control (100%), ###P ≤ .001 versus VEGF, and NS versus SKOV3/A2780. For A to E, data are presented as means ± SD. P values were calculated by one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. (F) Shotgun proteomics of SKOV3 CM by the <t>4800</t> <t>MALDI</t> TOF/TOF analyzer. Significant results were determined by selecting proteins that matched with two or more peptides with total ion CIs of >95%, followed by a literature search to determine proteins with a possible role in angiogenesis. (G) Analysis of CL, CD, IGFBP-7, and VEGF concentration in CM from SKOV3 and A2870. Commercially available ELISAs were used for determination of VEGF, CD, and CL concentrations, whereas the IGFBP-7 ELISA protocol was developed in-house. All experiments were carried out in duplicate on three separate samples. B/D, below detection limit.
4800 Maldi Tof/Tof Analyzer, supplied by Shotgun Proteomics company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mass spectrometers  (Thermo Fisher)
99
Thermo Fisher mass spectrometers
Ovarian cancer cell secretome increases HOMEC cellular responses and contains a range of potential proangiogenic proteins. (A) Proliferation of HOMECs treated with CM from SKOV3 and A2780 cells for 24, 48, or 72 hours assessed using the WST-1 assay. Controls received basal medium only. **P ≤ .01 and ***P ≤ .001 versus control levels (100%). (B) Migration of HOMECs in co-culture with ovarian cancer cells and HDFs (co-culture control) assessed by the ThinCert migration assay. After 24 hours, fluorescence of migrated cells was quantified. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus HDFs. (C) HOMEC Oris cell migration after 48 hours in the presence of VEGFA165 (20 ng/ml) ± SU5416 (10 µM). Controls received medium alone. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus VEGFA165. (D) Migration of HOMECs in co-culture with ovarian cancer cells ± anti-VEGFA antibody (500 ng/ml) or SU5416 (10 µM) assessed by the ThinCert migration assay. ***P ≤ .001 versus control (100%), **P ≤ .01 versus control (100%), and NS versus A2780 or SKOV3. (E) VEGF- and EOC cell-induced tube-like structure formation of HOMECs and the effects of the SU5416 inhibitor. HOMECs were plated onto fibrin matrices and exposed to either VEGFA165 or EOC cells in co-culture ± SU5416 (10 µM). Negative controls received medium alone. Tube-like structure formation was quantified. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 versus control (100%), ###P ≤ .001 versus VEGF, and NS versus SKOV3/A2780. For A to E, data are presented as means ± SD. P values were calculated by one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. (F) Shotgun proteomics of SKOV3 CM by the <t>4800</t> <t>MALDI</t> TOF/TOF analyzer. Significant results were determined by selecting proteins that matched with two or more peptides with total ion CIs of >95%, followed by a literature search to determine proteins with a possible role in angiogenesis. (G) Analysis of CL, CD, IGFBP-7, and VEGF concentration in CM from SKOV3 and A2870. Commercially available ELISAs were used for determination of VEGF, CD, and CL concentrations, whereas the IGFBP-7 ELISA protocol was developed in-house. All experiments were carried out in duplicate on three separate samples. B/D, below detection limit.
Mass Spectrometers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mass+spectrometer+4800+maldi+tof/Phosphate/pmc04780753-64-16-18
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Image Search Results


Ovarian cancer cell secretome increases HOMEC cellular responses and contains a range of potential proangiogenic proteins. (A) Proliferation of HOMECs treated with CM from SKOV3 and A2780 cells for 24, 48, or 72 hours assessed using the WST-1 assay. Controls received basal medium only. **P ≤ .01 and ***P ≤ .001 versus control levels (100%). (B) Migration of HOMECs in co-culture with ovarian cancer cells and HDFs (co-culture control) assessed by the ThinCert migration assay. After 24 hours, fluorescence of migrated cells was quantified. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus HDFs. (C) HOMEC Oris cell migration after 48 hours in the presence of VEGFA165 (20 ng/ml) ± SU5416 (10 µM). Controls received medium alone. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus VEGFA165. (D) Migration of HOMECs in co-culture with ovarian cancer cells ± anti-VEGFA antibody (500 ng/ml) or SU5416 (10 µM) assessed by the ThinCert migration assay. ***P ≤ .001 versus control (100%), **P ≤ .01 versus control (100%), and NS versus A2780 or SKOV3. (E) VEGF- and EOC cell-induced tube-like structure formation of HOMECs and the effects of the SU5416 inhibitor. HOMECs were plated onto fibrin matrices and exposed to either VEGFA165 or EOC cells in co-culture ± SU5416 (10 µM). Negative controls received medium alone. Tube-like structure formation was quantified. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 versus control (100%), ###P ≤ .001 versus VEGF, and NS versus SKOV3/A2780. For A to E, data are presented as means ± SD. P values were calculated by one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. (F) Shotgun proteomics of SKOV3 CM by the 4800 MALDI TOF/TOF analyzer. Significant results were determined by selecting proteins that matched with two or more peptides with total ion CIs of >95%, followed by a literature search to determine proteins with a possible role in angiogenesis. (G) Analysis of CL, CD, IGFBP-7, and VEGF concentration in CM from SKOV3 and A2870. Commercially available ELISAs were used for determination of VEGF, CD, and CL concentrations, whereas the IGFBP-7 ELISA protocol was developed in-house. All experiments were carried out in duplicate on three separate samples. B/D, below detection limit.

Journal: Translational Oncology

Article Title: Epithelial Ovarian Cancer-Induced Angiogenic Phenotype of Human Omental Microvascular Endothelial Cells May Occur Independently of VEGF Signaling 1 2

doi:

Figure Lengend Snippet: Ovarian cancer cell secretome increases HOMEC cellular responses and contains a range of potential proangiogenic proteins. (A) Proliferation of HOMECs treated with CM from SKOV3 and A2780 cells for 24, 48, or 72 hours assessed using the WST-1 assay. Controls received basal medium only. **P ≤ .01 and ***P ≤ .001 versus control levels (100%). (B) Migration of HOMECs in co-culture with ovarian cancer cells and HDFs (co-culture control) assessed by the ThinCert migration assay. After 24 hours, fluorescence of migrated cells was quantified. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus HDFs. (C) HOMEC Oris cell migration after 48 hours in the presence of VEGFA165 (20 ng/ml) ± SU5416 (10 µM). Controls received medium alone. ***P ≤ .001 versus control (100%) and ###P ≤ .001 versus VEGFA165. (D) Migration of HOMECs in co-culture with ovarian cancer cells ± anti-VEGFA antibody (500 ng/ml) or SU5416 (10 µM) assessed by the ThinCert migration assay. ***P ≤ .001 versus control (100%), **P ≤ .01 versus control (100%), and NS versus A2780 or SKOV3. (E) VEGF- and EOC cell-induced tube-like structure formation of HOMECs and the effects of the SU5416 inhibitor. HOMECs were plated onto fibrin matrices and exposed to either VEGFA165 or EOC cells in co-culture ± SU5416 (10 µM). Negative controls received medium alone. Tube-like structure formation was quantified. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 versus control (100%), ###P ≤ .001 versus VEGF, and NS versus SKOV3/A2780. For A to E, data are presented as means ± SD. P values were calculated by one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. (F) Shotgun proteomics of SKOV3 CM by the 4800 MALDI TOF/TOF analyzer. Significant results were determined by selecting proteins that matched with two or more peptides with total ion CIs of >95%, followed by a literature search to determine proteins with a possible role in angiogenesis. (G) Analysis of CL, CD, IGFBP-7, and VEGF concentration in CM from SKOV3 and A2870. Commercially available ELISAs were used for determination of VEGF, CD, and CL concentrations, whereas the IGFBP-7 ELISA protocol was developed in-house. All experiments were carried out in duplicate on three separate samples. B/D, below detection limit.

Article Snippet: P values were calculated by one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. (F) Shotgun proteomics of SKOV3 CM by the 4800 MALDI TOF/TOF analyzer.

Techniques: WST-1 Assay, Control, Migration, Co-Culture Assay, Fluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay